《生物反應(yīng)器課件Newtechnologiesincellculture.ppt》由會員分享，可在線閱讀，更多相關(guān)《生物反應(yīng)器課件Newtechnologiesincellculture.ppt（34頁珍藏版）》請?jiān)谘b配圖網(wǎng)上搜索。
1、New technologies in cell culture,Content,Introduction Comparison Sensor technologies,Introduction - bioreactors,Introduction - bioreactors,Capable of being operated aseptically for a number of days, weeks and even months Capable of generating repeatable process conditions for a number of days, weeks
2、 and even months Adequate aeration and agitation should be provided to meet the metabolic requirements of the micro-organism or cell pH, Oxygen, Temperature and other control should be provided Sampling facilities should be provided Should be suitable for a range of processes Should be constructed t
3、o ensure smooth internal surfaces (cleanability),-Flask,Test Tubes, Shake flasks. Increased aeration. Reduced evaporative loss. Solid support , Re-useable / Autoclavable. May be used with the SensorDish Reader.,-Flasks are Shake Flasks,-Flask ConfigurationsFrom 100ul to 4.0ml,SensorDish Reader , SDR
4、,SensorDish Reader , SDR,Non-invasive, online. pH or Dissolved Oxygen. No Calibration Required.,6-well,24-well,Expandable,Additional SDRs link into the Basic Set. Up to 10 SDRs on one Laptop. No additional software required for expansion.,SDRs Integrate with -Flasks,-Flasks Integrate with the Sensor
5、DishReader,Eliminate Well-plate inconsistency. Reduce and equalize evaporative losses across well-plates. Obtain online pH or dissolved oxygen data for all wells.,DO profiles (all 24 for each well-plate),Polystyrene,u-Flask,SensorFlaskReader , SFR,The SensorFlask Reader, SFR,Shaker-mounting blue-too
6、th sensor platform. Shake Flasks with DO and pH sensors. (also single DO or pH if desired) Multiple volumes (125/250/500/1000 ml). Up to 9 flasks on the platform at one time.,Microbial and Mammalian Applications,Microbial Applications: Monitor pH and DO levels. Ensure aerobic growth. Follow carbon s
7、ource utilization and exhaustion. Adjust orbiter speeds to maintain DO levels for better scalability.,Mammalian Applications: Accurately monitor pH /pCO2 levels. Adjust bicarbonate levels more accurately. Monitor DO levels, adjust orbiter speeds for better scalability.,Software,Individual Flask Cali
8、bration,Complete Hardware,Hardware Includes: Platform. Batteries. Bluetooth adapter. Software. Clamps. Bluetooth range of 15ft. Battery life 3 months on a single charge. (platform can operate on single battery),The RAMBIO1000 (Resonant Acoustic Mixing),Resonant Acoustic Mixing,All energy goes into m
9、ixing the contents of the flask 6 fold better OTRs than orbital shakers due to increased headspace exchange Up to 50% fill volume in standard flasks,The Applikon RAM,Accessories: Clamps and Oxy-pump Stoppers,250ml,1000ml,Single use applications,Small scale options, more R&D AppliFlex CellReady,Senso
10、r technologies,Traditional sensor pH and DO Sterilized via autoclave,Sensor technologies - Fluorescent,Fluorescent light is measure for pH and DO,Fluorescence pro/con,Pre-sterilized Pre-calibrated (pH) Single use + reusable (DO) Measurement through wall of vessel,pH range limited Not backwards compa
11、tible (controller) Can be influenced by medium (pH) Additional cost,PRO,CON,New technologies,AppliFlex reusableAppliFlex single sensorsuse sensor,New technologies,Reusable standard Single sensors use Sensor optional Ez-Control,Small scale bioreactors,Small scale bioreactor system,29,Small scale bioreactor,Communication via web browser Software to do calibration/setpoints/ control loops on multiple systems simultaneously Parallel cultivations?,BioSep Acoustic cell separation,BioSep,BioSep,Questions ?,
生物反應(yīng)器課件Newtechnologiesincellculture.ppt
